Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration.

The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal whole-mounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm2) and of the size of the neuronal cell bodies did not reveal statistically significant differences between the groups, but there was a greater incidence of large neurons in the protein restriction group (RP). The duodenum was markedly smaller in the RP group and, although there was no difference in the thickness of its wall, the mucosa was larger and the muscular layer was smaller in group RP. It was concluded that the neuronal and non-neuronal components of the duodenum adjust themselves to the nutritional condition, assuring the maintenance of their functions.

Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration.

The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22

) and hypoproteic (8

) rations, killed at the age of 345 days. For neuronal assessments duodenal whole-mounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm2) and of the size of the neuronal cell bodies did not reveal statistically significant differences between the groups, but there was a greater incidence of large neurons in the protein restriction group (RP). The duodenum was markedly smaller in the RP group and, although there was no difference in the thickness of its wall, the mucosa was larger and the muscular layer was smaller in group RP. It was concluded that the neuronal and non-neuronal components of the duodenum adjust themselves to the nutritional condition, assuring the maintenance of their functions.

Profile of the cell body of nadh-diaphorase positive myenteric neurons from the stomach of rats (Rattus norvegicus) subjected to chronic alcoholism - DOI: 10.4025/actascibiolsci.v26i3.1599 / Perfil do corpo celular de neurônios mioentéricos nadh-diaforase positivos do estômago de ratos (Rattus norvegicus) submetidos ao alcoolismo crônico - DOI: 10.4025/actascibiolsci.v26i3.1599

The purpose of this work was to compare the profile of the cell body of the NADH-diaphorase positive myenteric neurons from the stomach of rats. It was used 10 animals (Rattus norvegicus), from groups a) control (n = 5), that during 210 days had ad libitum supply of chow with normal protein level (22%) and water; and b) experimental (n = 5), that during 210 days had ad libitum supply of chow with normal protein level (22%) and sugar cane brandy diluted to 30 Gay Lussac (30o v/v). The stomachs collected and subjected to the technique for neuronal staining. The cell body of the neurons (n = 1.000) was measured through a computerized system of image analysis. The profile of the neurons from the control rats ranged from 60.16 and 638.64 µm2. In the experimental group the values ranged from 40.84 to 599.15 µm2. We observed a significant decrease on the cell body size, increase of the small neurons and decrease of the large neurons

O objetivo do trabalho foi comparar o perfil dos corpos celulares dos neurônios mioentéricos NADH-diaforase positivos do estômago de ratos. Utilizou-se 10 animais (Rattus norvegicus), provenientes dos grupos: a) controle (n=5), que durante 210 dias receberam, ad libitum, dieta com teor protéico normal (22%) e água; e b) experimental (n = 5), que durante 210 dias receberam, ad libitum, ração com teor protéico normal (22%) e aguardente-de-cana diluída a 30 Gay Lussac (30º v/v). Os estômagos coletados foram submetidos à técnica de evidenciação neuronal. A mensuração do perfil dos corpos celulares dos neurônios (n = 1.000) foi através de um Sistema Computadorizado de Análise de Imagem. O perfil dos corpos celulares dos neurônios do grupo controle ficou entre 60,16 a 638,64 µm2. No grupo experimental variou de 40,84 a 599,15 µm2. Constatamos redução significante no tamanho do corpo celular, aumento de neurônios pequenos e diminuição de neurônios grandes

Morphoquantitative study of the duodenum myenteric plexus from rats subjected to lack of proteic and complex B vitamins - DOI: 10.4025/actascibiolsci.v26i2.1642 / Estudo morfoquantitativo do plexo mioentérico do duodeno de ratos submetidos a carência de proteínas e vitaminas do complexo B - DOI: 10.4025/actascibiolsci.v26i2.1642

This study aims to evaluate the effects of protein and vitamin deficiency on the neurons of the duodenum myenteric plexus of rats. 24 90-day old animals were divided in two groups: control (n=12) and experimental (n=12). During 120 days, the control group received chow with 22% protein level, vitamins and minerals; the experimental group was fed with 8%-protein chow, without vitamin supplementation. After the experimental period, the animals blood was collected and the duodenum was removed for ultrastructural and morphoquantitative analyses (NADH-d; NADPH-d). The experimental group had reduced total protein, plasma albumin and body weight lower than the control group. The density (NADPH-d) was higher and significant for the experimental group; with the NADH-d technique the neuronal profile differed between the groups. The ultrastructural aspects were similar in both groups. The results allow the conclusion that the diet caused a moderate deficiency, not leading to quantitative alterations, but changed the neuronal profile

Este estudo teve como objetivo avaliar os efeitos das carências protéica e vitamínica sobre os neurônios do plexo mioentérico do duodeno de ratos. 24 animais, aos 90 dias de idade, foram divididos em dois grupos: controle (n=12) e experimental (n=12). Durante 120 dias, o grupo controle recebeu ração com teor protéico de 22%, vitaminas e minerais; o grupo experimental, 8%, sem suplementação de vitaminas. Após período experimental, coletou-se o sangue dos animais e retirou-se o duodeno para análise ultraestrutural e morfoquantitativa (NADH-d; NADPH-d). O grupo experimental apresentou proteínas totais e albumina plasmática reduzidas e peso inferior aos do grupo controle. A densidade (NADPH-d) foi maior e significante para o grupo experimental; na técnica da NADH-d, o perfil neuronal diferiu entre os grupos. Os aspectos ultraestruturais mostraram-se semelhantes entre os grupos. Os resultados permitem concluir que a dieta imposta promoveu um quadro moderado de desnutrição, não provocando alterações quantitativas, porém levou a alterações no perfil neuronal

Evaluation of the population of NADPH-diaphorase positive myenteric neurons from the body of the stomach of diabetic rats induced by streptozotocin - DOI: 10.4025/actascibiolsci.v26i1.1667 / Avaliação da população de neurônios mioentéricos NADPH-diaforase positivos do corpo do estômago de ratos com diabetes crônico induzido pela estreptozootocina - DOI: 10.4025/actascibiolsci.v26i1.1667

The aim of this study was to analyze the morphoquantitative features of the nitrergic myenteric neurons from the body of the stomach of diabetic rats. The body of the stomach of five normoglicemic rats and of five diabetic rats were prepared as whole-mounts stained by the histochemical technique of NADPH-diaphorase. Decreased body weight and increased daily ingestion of water, fast glicemia and glycated hemoglobin were verified in diabetic animals. According to the data obtained, significant difference in the density of nitrergic neurons was not observed between the two groups, but the areas of the neuronal cell body profiles in the diabetic rats were significantly larger. Results showed that the streptozotocin that induced diabetes does not accelerate the death of the nitrergic neurons, but increases the expression of these cells

O presente estudo teve por objetivo analisar as características morfoquantitativas de neurônios mioentéricos nitrérgicos do corpo do estômago de ratos diabéticos. O corpo do estômago de 5 ratos normoglicêmicos e de 5 ratos diabéticos foi submetido a preparados de membrana corados pela técnica histoquímica da NADPH-diaforase. Verificaram-se, nos animais diabéticos, diminuição do peso corporal, aumento do consumo diário de água, da glicemia em jejum e da hemoglobina glicada. Com os dados obtidos, não foi observada diferença significativa na densidade de neurônios nitrérgicos entre os dois grupos, porém as áreas dos perfis celulares neuronais dos ratos diabéticos foram significativamente maiores. Constatou-se, portanto, que o diabetes induzido por estreptozootocina não acelera a morte dos neurônios nitrérgicos, contudo aumenta a expressão desses neurônios